Improved Monosynaptic Neural Circuit Tracing Using Engineered Rabies Virus Glycoproteins.

@article{Kim2016ImprovedMN,
  title={Improved Monosynaptic Neural Circuit Tracing Using Engineered Rabies Virus Glycoproteins.},
  author={Euiseok J. Kim and Matthew W. Jacobs and Tony Ito-Cole and Edward M. Callaway},
  journal={Cell reports},
  year={2016},
  volume={15 4},
  pages={692-699}
}
Monosynaptic rabies virus tracing is a unique and powerful tool used to identify neurons making direct presynaptic connections onto neurons of interest across the entire nervous system. Current methods utilize complementation of glycoprotein gene-deleted rabies of the SAD B19 strain with its glycoprotein, B19G, to mediate retrograde transsynaptic spread across a single synaptic step. In most conditions, this method labels only a fraction of input neurons and would thus benefit from improved… CONTINUE READING
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