Improved Methods for Immunoassay of Mycothiol

@article{Unson1999ImprovedMF,
  title={Improved Methods for Immunoassay of Mycothiol},
  author={M. D. Unson and G. Newton and K. Arnold and C. Davis and R. C. Fahey},
  journal={Journal of Clinical Microbiology},
  year={1999},
  volume={37},
  pages={2153 - 2157}
}
ABSTRACT Improved enzyme-linked immunosorbent assay (ELISA) methods have been developed for the determination of femtomole amounts of mycothiol (MSH), the main low-molecular-weight thiol in mycobacteria. The immunoassays utilize an affinity-purified rabbit polyclonal antibody that is highly specific for the pseudodisaccharide moiety of MSH. MSH was first biotinylated by the thiol-specific reagent 3-(N-maleimidopropionyl)biocytin. The MSH-biotin adduct was then captured with immobilized avidin… Expand
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