Improved Immunohistochemical Protocol for Organotypic Brain Slice Cultures

@inproceedings{Yoon2010ImprovedIP,
  title={Improved Immunohistochemical Protocol for Organotypic Brain Slice Cultures},
  author={Jinny Jung Yoon and Vithika Suri and Louise Frances Basford Nicholson and Colin Richard Green},
  year={2010}
}
This study aimed to improve existing immunohistochemical methods in order to obtain continuous multi-fluorescence labelling throughout the full thickness of cultured hippocampal slices whilst maintaining structural integrity. Slice cultures, processed as whole mounts for triple immunofluorescence labelling, were examined using a confocal laser scanning microscope. The thickness of the mounted slices and penetration of antibodies were investigated using conventional and bridge mounting… CONTINUE READING