Improved Human Pluripotent Stem Cell Attachment and Spreading on Xeno-Free Laminin-521-Coated Microcarriers Results in Efficient Growth in Agitated Cultures

@inproceedings{Lam2015ImprovedHP,
  title={Improved Human Pluripotent Stem Cell Attachment and Spreading on Xeno-Free Laminin-521-Coated Microcarriers Results in Efficient Growth in Agitated Cultures},
  author={Alan Tin-Lun Lam and Jian Li and Allen Kuan-Liang Chen and William R Birch and Shaul Reuveny and Steve Kah-Weng Oh},
  booktitle={BioResearch open access},
  year={2015}
}
Human pluripotent stem cells (hPSC) are self-renewing cells having the potential of differentiation into the three lineages of somatic cells and thus can be medically used in diverse cellular therapies. One of the requirements for achieving these clinical applications is development of completely defined xeno-free systems for large-scale cell expansion and differentiation. Previously, we demonstrated that microcarriers (MCs) coated with mouse laminin-111 (LN111) and positively charged poly-l… CONTINUE READING

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