Improved HPLC method for the determination of curcumin, demethoxycurcumin, and bisdemethoxycurcumin.

@article{Jayaprakasha2002ImprovedHM,
  title={Improved HPLC method for the determination of curcumin, demethoxycurcumin, and bisdemethoxycurcumin.},
  author={Guddadarangavvanahally K Jayaprakasha and Lingamullu Jagan Mohan Rao and Kunnumpurath K Sakariah},
  journal={Journal of agricultural and food chemistry},
  year={2002},
  volume={50 13},
  pages={
          3668-72
        }
}
Commercially available curcumin, a bright orange-yellow color pigment of turmeric, consists of a mixture of three curcuminoids, namely, curcumin, demethoxycurcumin, and bisdemethoxycurcumin. These were isolated by column chromatography and identified by spectroscopic studies. The purity of the curcuminoids was analyzed by an improved HPLC method. HPLC separation was performed on a C(18) column using three solvents, methanol, 2% AcOH, and acetonitrile, with detection at 425 nm. Four different… CONTINUE READING

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