Improved Dual-Luciferase Reporter Assays for Nuclear Receptors

@inproceedings{Paguio2010ImprovedDR,
  title={Improved Dual-Luciferase Reporter Assays for Nuclear Receptors},
  author={Aileen Paguio and Pete Stecha and Keith V. Wood and Frank Fan},
  booktitle={Current chemical genomics},
  year={2010}
}
Nuclear receptors play important roles in many cellular functions through control of gene transcription. It is also a large target class for drug discovery. Luciferase reporter assays are frequently used to study nuclear receptor function because of their wide dynamic range, low endogenous activity, and ease of use. Recent improvements of luciferase genes and vectors have further enhanced their utilities. Here we applied these improvements to two reporter formats for studying nuclear receptors… CONTINUE READING

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