Improved Bioconversion of 15‐Fluoro‐6‐deoxyerythronolide B to 15‐Fluoro‐erythromycin A by Overexpression of the eryK Gene in Saccharopolyspora erythraea

@article{Desai2004ImprovedBO,
  title={Improved Bioconversion of 15‐Fluoro‐6‐deoxyerythronolide B to 15‐Fluoro‐erythromycin A by Overexpression of the eryK Gene in Saccharopolyspora erythraea},
  author={R. Desai and E. Rodriguez and J. Galazzo and P. Licari},
  journal={Biotechnology Progress},
  year={2004},
  volume={20}
}
The bioconversion of a 6‐deoxyerythronolide B analogue to the corresponding erythromycin A analogue (R‐EryA) by a Saccharopolyspora erythraea mutant lacking the ketosynthase in the first polyketide synthase module was significantly improved by changing fluxes at a key branch point affecting the erythromycin congener distribution. This was achieved by integrating an additional copy of the eryK gene into the chromosome under control of the eryAIp promoter. Real‐time PCR analysis of RNA confirmed… Expand
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