Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys

@inproceedings{Walters2016ImprovedB1,
  title={Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys},
  author={William Anton Walters and Embriette R. Hyde and Donna Berg-Lyons and Gail Ackermann and Greg C. Humphrey and Alma E Parada and Jack A. Gilbert and Janet K. Jansson and J. Gregory Caporaso and Jed A. Fuhrman and Amy Apprill and Rob Knight},
  booktitle={mSystems},
  year={2016}
}
Designing primers for PCR-based taxonomic surveys that amplify a broad range of phylotypes in varied community samples is a difficult challenge, and the comparability of data sets amplified with varied primers requires attention. Here, we examined the performance of modified 16S rRNA gene and internal transcribed spacer (ITS) primers for archaea/bacteria and fungi, respectively, with nonaquatic samples. We moved primer bar codes to the 5' end, allowing for a range of different 3' primer… CONTINUE READING
Recent Discussions
This paper has been referenced on Twitter 178 times over the past 90 days. VIEW TWEETS
56 Citations
19 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 56 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 19 references

Similar Papers

Loading similar papers…