Importance of two buried salt bridges in the stability and folding pathway of barnase.

@article{Tissot1996ImportanceOT,
  title={Importance of two buried salt bridges in the stability and folding pathway of barnase.},
  author={Ana Cristina Tissot and St{\'e}phane Vuilleumier and A. R. Fersht},
  journal={Biochemistry},
  year={1996},
  volume={35 21},
  pages={
          6786-94
        }
}
The importance of two buried salt bridges in barnase in the stability of its folded state, the major transition rate for unfolding, and a folding intermediate has been analyzed by protein engineering, kinetic, and thermodynamic studies. The aspartate residues in the bridges Arg69-Asp93 and Arg83-Asp75 were replaced by the isosteric analogue asparagine, while various replacements were probed for the positively charged arginine partners. The mutations are very destabilizing, lowering stability by… CONTINUE READING

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