Importance of phosphate contacts for sequence recognition by EcoRI restriction enzyme.

@article{Rosati2002ImportanceOP,
  title={Importance of phosphate contacts for sequence recognition by EcoRI restriction enzyme.},
  author={Olaf Rosati and Tushar K Srivastava and Seturam B Katti and J{\"u}rgen Alves},
  journal={Biochemical and biophysical research communications},
  year={2002},
  volume={295 1},
  pages={198-205}
}
We have studied the importance of charge and hydrogen-bonding potential of the phosphodiester backbone for binding and cleavage by EcoRI restriction endonuclease. We used 12-mer oligodeoxynucleotide substrates with single substitutions of phosphates by chiral methylphosphonates at each position of the recognition sequence -pGpApApTpTpCp-. Binding was moderately reduced between 4- and 400-fold more or less equally for the R(P) and S(P)-analogues mainly caused by missing charge interaction. The… CONTINUE READING