Importance of methane-oxidizing bacteria in the methane budget as revealed by the use of a specific inhibitor

  title={Importance of methane-oxidizing bacteria in the methane budget as revealed by the use of a specific inhibitor},
  author={Ronald S. Oremland and Charles W. Culbertson},
METHANE is a greenhouse gas whose concentration in the atmosphere is increasing1–3. Much of this methane is derived from the metabolism of methane-generating (methanogenic) bacteria4,5 and over the past two decades much has been learned about the ecology of methanogens; specific inhibitors of methanogenesis, such as 2-bromoethanesulphonic acid, have proved useful in this regard6. In contrast, although much is known about the biochemistry of methane-oxidizing (methanotrophic) bacteria7… Expand
Evaluation of alternative substrates for determining methane-oxidizing activities and methanotrophic populations in soils
The magnitude of methane emission is a net result of methane production and the oxidation rate. The possibility of measuring oxidized products of alternative substrates of methane monooxygenase wasExpand
Effects of Nitrogen Load on the Function and Diversity of Methanotrophs in the Littoral Wetland of a Boreal Lake
The overall activity of a methanotroph community in littoral wetlands is not affected by nitrogen leached from the catchment area, so the effects of nitrogen on metanotrophic activity are unknown. Expand
Methane-oxidizing activities and methanotrophic populations associated with wetland rice plants
Abstract Acetylene up to 500 μl l–1 did not affect methane formation in anoxic soil up to 12 h, but further incubation for 1 week showed strong inhibition of methanogenesis. To ascertain the extentExpand
Aerobic Methanotrophy and Nitrification: Processes and Connections
Ammonia and methane are structurally similar molecules. Not surprisingly therefore, microorganisms that use methane as a sole energy source (methanotrophs) and microorganisms that use ammonia as aExpand
Aerobic H2 respiration enhances metabolic flexibility of methanotrophic bacteria
It is shown that a methanotrophic bacterium from the phylum Verrucomicrobia oxidises hydrogen gas (H2) during growth and persistence, and it is proposed that H2 is consumed by this bacterium for mixotrophicgrowth and persistence in a manner similar to other non-methanotrophic soil microorganisms. Expand
Methanotrophs and copper.
The current state of knowledge of the phylogeny, environmental distribution, and potential applications of methanotrophs for regional and global issues are summarized, as well as the role of Cu in regulating gene expression and proteome in these cells, its effects on enzymatic and whole-cell activity, and the novel Cu uptake system used by methanOTrophs are summarized. Expand
Genomic and Physiological Properties of a Facultative Methane-Oxidizing Bacterial Strain of Methylocystis sp. from a Wetland
It was revealed that strain B8 might survive atmospheric methane concentration and had various genes for hydrogenase, nitrogen fixation, polyhydroxybutyrate synthesis, and heavy metal resistance, which might enable its survival in wetland environments. Expand
Exploration and enrichment of methane-oxidizing bacteria derived from a rice paddy field emitting highly concentrated methane.
The quantification of functional genes encoding methane monooxygenase, pmoA and PHA synthase, phaC, by quantitative PCR revealed concomitant increases in accordance with the Type II MOB enrichment, underscoring the significance of Type I MOB to mitigate greenhouse gas emission and produce PHA. Expand
Evaluation of potential inhibitors of methanogenesis and methane oxidation in a land ® ll cover soil
Biological methane (CH4) production is an anaerobic process, while CH4 consumption occurs predominantly under aerobic conditions; however, both processes can occur simultaneously in soil. Thus, ®eldExpand
Regulation of root-associated methanotrophy by oxygen availability in the rhizosphere of two aquatic macrophytes
The relative importance of oxygen for root-associated methanotrophy was examined by using sediment-free, intact freshwater marsh plants (Pontederia cordata and Sparganium eurycarpum) incubated in split chambers to compare their relative ability to oxygenate their rhizospheres. Expand


Methane production and simultaneous sulphate reduction in anoxic, salt marsh sediments
It has been generally believed that sulphate reduction precludes methane generation during diagenesis of anoxic sediments1,2. Because most biogenic methane formed in nature is thought to deriveExpand
Biogeochemical aspects of atmospheric methane
Methane is the most abundant organic chemical in Earth's atmosphere, and its concentration is increasing with time, as a variety of independent measurements have shown. Photochemical reactionsExpand
Regulation by light of methane emissions from a wetland
WETLANDS provide up to 25% of the annual global flux of methane—an important greenhouse gas—to the atmosphere1–3. Despite many studies4–9, however, the factors that control emission from wetlands areExpand
The importance of methane and thiosulfate in the metabolism of the bacterial symbionts of two deep-sea mussels
The methanotrophic nature of the seep-mussel symbionts was confirmed in 14C-methane uptake experiments by the appearance of label in both CO2 and acid-stable, non-volatile, organic compounds after a 3 h incubation of isolated gill tissue, and methane consumption was correlated with methanol dehydrogenase activity in isolated gills tissue. Expand
Use of “Specific” Inhibitors in Biogeochemistry and Microbial Ecology
The term “specific inhibitor” has been applied to these types of compounds when they are used to probe the functions of mixed populations of microorganisms, providing powerful experimental tools for investigating the activity and function of certain types of micro organisms in natural samples. Expand
Nitrogen Fixation Dynamics of Two Diazotrophic Communities in Mono Lake, California
  • R. Oremland
  • Biology, Medicine
  • Applied and environmental microbiology
  • 1990
This article corrects the article on p. 614 in vol. Expand
Consumption of atmospheric methane by tundra soils
EMISSION of methane from tundra soil contributes about 10% of the global atmospheric methane budget1. Moreover, tundra soils contain 15% of global soil carbon2, so the response of this large carbonExpand
Carbon-14 in Methane Sources and in Atmospheric Methane: The Contribution from Fossil Carbon
Model calculations of source partitioning based on the carbon-14 data, CH4 concentrations, and δ13C in CH4 indicate that 21 � 3% of atmospheric CH4 was derived from fossil carbon at the end of 1987, and data indicate that pressurized water reactors are an increasingly important source of 14CH4. Expand
Hydrogen Metabolism by Decomposing Cyanobacterial Aggregates in Big Soda Lake, Nevada
  • R. Oremland
  • Chemistry, Medicine
  • Applied and environmental microbiology
  • 1983
Hydrogen production in these cyanobacterial communities appears to be caused by the resident bacterial flora and not by the cyanobacteria. Expand
Production, oxidation and emission of methane in rice paddies
Production and emission of methane from submerged paddy soil was studied in laboratory rice cultures and in Italian paddy fields. Up to 80% of the CH4 produced in the paddy soil did not reach theExpand