Importance of methane-oxidizing bacteria in the methane budget as revealed by the use of a specific inhibitor

  title={Importance of methane-oxidizing bacteria in the methane budget as revealed by the use of a specific inhibitor},
  author={Ronald S. Oremland and Charles W. Culbertson},
METHANE is a greenhouse gas whose concentration in the atmosphere is increasing1–3. Much of this methane is derived from the metabolism of methane-generating (methanogenic) bacteria4,5 and over the past two decades much has been learned about the ecology of methanogens; specific inhibitors of methanogenesis, such as 2-bromoethanesulphonic acid, have proved useful in this regard6. In contrast, although much is known about the biochemistry of methane-oxidizing (methanotrophic) bacteria7… 

Effects of Nitrogen Load on the Function and Diversity of Methanotrophs in the Littoral Wetland of a Boreal Lake

The overall activity of a methanotroph community in littoral wetlands is not affected by nitrogen leached from the catchment area, so the effects of nitrogen on metanotrophic activity are unknown.

Methane-oxidizing activities and methanotrophic populations associated with wetland rice plants

Abstract Acetylene up to 500 μl l–1 did not affect methane formation in anoxic soil up to 12 h, but further incubation for 1 week showed strong inhibition of methanogenesis. To ascertain the extent

Aerobic Methanotrophy and Nitrification: Processes and Connections

Microbial ecologists have attempted to find specific inhibitors for either group in order to study their respective roles in the environment, and the contribution of ammonia oxidisers to methanotrophy in natural systems appears to be very minor.

Aerobic H2 respiration enhances metabolic flexibility of methanotrophic bacteria

It is shown that a methanotrophic bacterium from the phylum Verrucomicrobia oxidises hydrogen gas (H2) during growth and persistence, and it is proposed that H2 is consumed by this bacterium for mixotrophicgrowth and persistence in a manner similar to other non-methanotrophic soil microorganisms.

Methanotrophs and copper.

The current state of knowledge of the phylogeny, environmental distribution, and potential applications of methanotrophs for regional and global issues are summarized, as well as the role of Cu in regulating gene expression and proteome in these cells, its effects on enzymatic and whole-cell activity, and the novel Cu uptake system used by methanOTrophs are summarized.

Genomic and Physiological Properties of a Facultative Methane-Oxidizing Bacterial Strain of Methylocystis sp. from a Wetland

It was revealed that strain B8 might survive atmospheric methane concentration and had various genes for hydrogenase, nitrogen fixation, polyhydroxybutyrate synthesis, and heavy metal resistance, which might enable its survival in wetland environments.

Evaluation of potential inhibitors of methanogenesis and methane oxidation in a land ® ll cover soil

Biological methane (CH4) production is an anaerobic process, while CH4 consumption occurs predominantly under aerobic conditions; however, both processes can occur simultaneously in soil. Thus, ®eld

Rhizospheric methane oxidation determined via the methyl fluoride inhibition technique

Methane oxidation rates in the rhizosphere of aquatic macrophytes were quantified by development of a technique employing a recently described inhibitor of methane oxidation, methyl fluoride. Unlike

Methanol Promotes Atmospheric Methane Oxidation by Methanotrophic Cultures and Soils

The results suggest that methanol or other nonmethane substrates may promote atmospheric methane oxidation in situ.



Methane production and simultaneous sulphate reduction in anoxic, salt marsh sediments

It has been generally believed that sulphate reduction precludes methane generation during diagenesis of anoxic sediments1,2. Because most biogenic methane formed in nature is thought to derive

Regulation by light of methane emissions from a wetland

  • G. King
  • Environmental Science
  • 1990
WETLANDS provide up to 25% of the annual global flux of methane—an important greenhouse gas—to the atmosphere1–3. Despite many studies4–9, however, the factors that control emission from wetlands are

The importance of methane and thiosulfate in the metabolism of the bacterial symbionts of two deep-sea mussels

The methanotrophic nature of the seep-mussel symbionts was confirmed in 14C-methane uptake experiments by the appearance of label in both CO2 and acid-stable, non-volatile, organic compounds after a 3 h incubation of isolated gill tissue, and methane consumption was correlated with methanol dehydrogenase activity in isolated gills tissue.

Use of “Specific” Inhibitors in Biogeochemistry and Microbial Ecology

The term “specific inhibitor” has been applied to these types of compounds when they are used to probe the functions of mixed populations of microorganisms, providing powerful experimental tools for investigating the activity and function of certain types of micro organisms in natural samples.

Nitrogen Fixation Dynamics of Two Diazotrophic Communities in Mono Lake, California

  • R. Oremland
  • Environmental Science
    Applied and environmental microbiology
  • 1990
This article corrects the article on p. 614 in vol.

Consumption of atmospheric methane by tundra soils

EMISSION of methane from tundra soil contributes about 10% of the global atmospheric methane budget1. Moreover, tundra soils contain 15% of global soil carbon2, so the response of this large carbon

Carbon-14 in Methane Sources and in Atmospheric Methane: The Contribution from Fossil Carbon

Model calculations of source partitioning based on the carbon-14 data, CH4 concentrations, and δ13C in CH4 indicate that 21 � 3% of atmospheric CH4 was derived from fossil carbon at the end of 1987, and data indicate that pressurized water reactors are an increasingly important source of 14CH4.

Hydrogen Metabolism by Decomposing Cyanobacterial Aggregates in Big Soda Lake, Nevada

  • R. Oremland
  • Environmental Science, Biology
    Applied and environmental microbiology
  • 1983
Hydrogen production in these cyanobacterial communities appears to be caused by the resident bacterial flora and not by the cyanobacteria.

Production, oxidation and emission of methane in rice paddies

Production and emission of methane from submerged paddy soil was studied in laboratory rice cultures and in Italian paddy fields. Up to 80% of the CH4 produced in the paddy soil did not reach the

Inhibition of methanogenesis in marine sediments by acetylene and ethylene: validity of the acetylene reduction assay for anaerobic microcosms.

Methanogenesis was irreversibly inhibited in sediments by concentrations of acetylene employed in nitrogen fixation assays (1 to 20%, vol/vol), and the inhibition was reversed by gassing with hydrogen.