Importance of co-cultivation medium pH for successful Agrobacterium-mediated transformation of Lilium × formolongi

  title={Importance of co-cultivation medium pH for successful Agrobacterium-mediated transformation of Lilium × formolongi},
  author={Mamoru Ogaki and Yukio Furuichi and Kiyo Kuroda and Dong Poh Chin and Yoichi Ogawa and Masahiro Mii},
  journal={Plant Cell Reports},
An efficient system for Agrobacterium-mediated transformation of Lilium × formolongi was established by preventing the drastic drop of pH in the co-cultivation medium with MES. Meristematic nodular calli were inoculated with an overnight culture of A. tumefaciens strain EHA101 containing the plasmid pIG121-Hm which harbored intron-containing β-glucuronidase (GUS), hygromycin phosphotransferase (HPT), and neomycin phosphotransfease II (NPTII) genes. After three days of co-cultivation on 2 g/l… CONTINUE READING
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