Importance of arginines 63 and 423 in modulating the bile salt-dependent and bile salt-independent hydrolytic activities of rat carboxyl ester lipase.

@article{Liang2000ImportanceOA,
  title={Importance of arginines 63 and 423 in modulating the bile salt-dependent and bile salt-independent hydrolytic activities of rat carboxyl ester lipase.},
  author={Yuyao Liang and Rohit Medhekar and Howard L. Brockman and Daniel M. Quinn and David Y Hui},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 31},
  pages={24040-6}
}
Previous studies using chemical modification approach have shown the importance of arginine residues in bile salt activation of carboxyl ester lipase (CEL) activity. However, the x-ray crystal structure of CEL failed to show the involvement of arginine residues in CEL-bile salt interaction. The current study used a site-specific mutagenesis approach to determine the role of arginine residues 63 and 423 in bile salt-dependent and bile salt-independent hydrolytic activities of rat CEL. Mutations… CONTINUE READING

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