Importance of L-selectin-dependent leukocyte-leukocyte interactions in human whole blood.

@article{Mitchell2000ImportanceOL,
  title={Importance of L-selectin-dependent leukocyte-leukocyte interactions in human whole blood.},
  author={Dennis J. Mitchell and Ping-Chia Li and Paul H. Reinhardt and Paul Kubes},
  journal={Blood},
  year={2000},
  volume={95 9},
  pages={2954-9}
}
The objective of this study was to investigate whether leukocytes could be recruited by rolling leukocytes in a human whole blood model system. In all experiments, either neutrophils, whole blood, or diluted blood was perfused over immobilized E-selectin. With isolated neutrophils (2 x 10(5)/mL), the free-flowing neutrophils were captured by attached neutrophils to form secondary interactions that resulted in lines of rolling leukocytes. These secondary tethers accounted for 50% to 60% of all… CONTINUE READING