Importance for Absorption of Na+ from Freshwater of Lysine, Valine and Serine Substitutions in the α1a-Isoform of Na,K-ATPase in the Gills of Rainbow Trout (Oncorhynchus mykiss) and Atlantic Salmon (Salmo salar)

@article{Jrgensen2008ImportanceFA,
  title={Importance for Absorption of Na+ from Freshwater of Lysine, Valine and Serine Substitutions in the $\alpha$1a-Isoform of Na,K-ATPase in the Gills of Rainbow Trout (Oncorhynchus mykiss) and Atlantic Salmon (Salmo salar)},
  author={P. L. J{\o}rgensen},
  journal={Journal of Membrane Biology},
  year={2008},
  volume={223},
  pages={37-47}
}
In the gills of rainbow trout and Atlantic salmon, the α1a- and α1b-isoforms of Na,K-ATPase are expressed reciprocally during salt acclimation. The α1a-isoform is important for Na+ uptake in freshwater, but the molecular basis for the functional differences between the two isoforms is not known. Here, three amino acid substitutions are identified in transmembrane segment 5 (TM5), TM8 and TM9 of the α1a-isoform compared to the α1b-isoform, and the functional consequences are examined by… Expand
Effects of seawater acclimation on two Na+/K+-ATPase α-subunit isoforms in the gills of the marble goby, Oxyeleotris marmorata.
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  • 2020
TLDR
The marble goby, Oxyeleotris marmorata, is a freshwater teleost, but can acclimate progressively to survive in seawater (salinity 30), and lacks specific freshwater- and seawater-type Nkaα isoforms as demonstrated by some other euryhaline fish species. Expand
Roles of three branchial Na(+)-K(+)-ATPase α-subunit isoforms in freshwater adaptation, seawater acclimation, and active ammonia excretion in Anabas testudineus.
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TLDR
Both seawater acclimation and ammonia exposure led to significant increases in the protein abundance and changes in the kinetic properties of branchial Na(+)-K(+-ATPase (Nka) α-subunit isoforms, but they involved two different types of Nka-immunoreactive cells. Expand
Differential expression of Na+, K+-ATPase α-1 isoforms during seawater acclimation in the amphidromous galaxiid fish Galaxias maculatus
TLDR
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Multiplicity of expression of Na+,K+–ATPaseα -subunit isoforms in the gill of Atlantic salmon (Salmo salar): cellular localisation and absolute quantification in response to salinity change
TLDR
The predominant switching of Na+,K+–ATPase α-subunit isoform mRNA during salinity acclimation reflects a marked remodelling of mitochondrion-rich cells (MRCs) in the gill and probably tuning of the pump performance to accomplish a net reversal of gill ion transport in hypo- and hypertonic environments. Expand
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FXYD-11 associates with Na+-K+-ATPase in the gill of Atlantic salmon: regulation and localization in relation to changed ion-regulatory status.
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TLDR
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Distinct hormonal regulation of Na(+),K(+)-atpase genes in the gill of Atlantic salmon (Salmo salar L.).
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Distinct freshwater and seawater isoforms of Na+/K+-ATPase in gill chloride cells of Atlantic salmon
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The results indicate that there is a freshwater and a seawater isoform of NKA α-subunit in the gills of Atlantic salmon and that they are present in distinct chloride cells. Expand
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It is shown that the Japanese medaka does not exhibit a salinity-induced α isoform switch and whether salinity affects ouabain, Mg2+, Na+ and K+ affinity of the gill enzyme, which suggests that Na+ affinity changes involve altered posttranslational modification or intermolecular interactions. Expand
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TLDR
Isoform switching in trout gills following salinity transfer suggests that the Na+/K+-ATPase α1a- andα 1b-isoforms play different roles in freshwater and seawater acclimation, and that assays of Na+/(K)+- ATPase enzyme activity may not provide a complete picture of the role of this protein in seawater transfer. Expand
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Findings demonstrate that landlocked salmon have lost some of the unique preparatory upregulation of gill NKA, NKCC and, to some extent, CFTR anion channel associated with the development of hypo-osmoregulatory ability in anadromous salmon. Expand
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The data suggest that rotating or tilting of the cytoplasmic part of the fifth transmembrane segment may adapt distances between coordinating groups and contribute to the distinctive Na+/K+ selectivity of the pump. Expand
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TLDR
Findings demonstrate that landlocked salmon have lost some of the unique preparatory upregulation of gill NKA, NKCC and, to some extent, CFTR anion channel associated with the development of hypo-osmoregulatory ability in anadromous salmon. Expand
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TLDR
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TLDR
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TLDR
It is contended that Na+-K+-ATPase partitions into SGC-enriched rafts to help facilitate the up-regulation of its activity during seawater adaptation and extended the working definition of cholesterol-dependent raft integrity to structural dependence on the sulfate moiety of SGC in this membrane. Expand
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