Import of mitochondrial transcription factor A (TFAM) into rat liver mitochondria stimulates transcription of mitochondrial DNA.

@article{Garstka2003ImportOM,
  title={Import of mitochondrial transcription factor A (TFAM) into rat liver mitochondria stimulates transcription of mitochondrial DNA.},
  author={Heike L. Garstka and Wolfgang E Schmitt and Jeanette E. Schultz and Bettina Sogl and Barbara Silakowski and Acisclo P{\'e}rez-Martos and Julio Montoya and Rudolf J Wiesner},
  journal={Nucleic acids research},
  year={2003},
  volume={31 17},
  pages={
          5039-47
        }
}
Mitochondrial transcription factor A (TFAM) has been shown to stimulate transcription from mitochondrial DNA promoters in vitro. In order to determine whether changes in TFAM levels also regulate RNA synthesis in situ, recombinant human precursor proteins were imported into the matrix of rat liver mitochondria. After uptake of wt-TFAM, incorporation of [alpha-32P]UTP into mitochondrial mRNAs as well as rRNAs was increased 2-fold (P < 0.05), whereas import of truncated TFAM lacking 25 amino… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 45 CITATIONS

References

Publications referenced by this paper.
SHOWING 1-10 OF 56 REFERENCES

Mitochondrial DNA maintenance in vertebrates.

  • Annual review of biochemistry
  • 1997
VIEW 7 EXCERPTS
HIGHLY INFLUENTIAL

The synthesis of mRNA in isolated mitochondria can be maintained for several hours and is inhibited by high levels of ATP

J. A. Enriquez, S. P. Fernandez, M. A. Perez, P. M. Lopez, J. Montoya
  • Eur. J. Biochem.,
  • 1996
VIEW 7 EXCERPTS
HIGHLY INFLUENTIAL