Import of alcohol oxidase into peroxisomes of Saccharomyces cerevisiae.

  title={Import of alcohol oxidase into peroxisomes of Saccharomyces cerevisiae.},
  author={B. Distel and Marten van der Klei Veenhuis and Henk F. Tabak},
  journal={The EMBO journal},
  volume={6 10},
Saccharomyces cerevisiae is unable to grow on methanol because it lacks the enzymes required for its metabolism. To study the possibility of whether or not the methanol oxidation pathway of Hansenula polymorpha can be transferred to S. cerevisiae, the gene coding for alcohol oxidase, a peroxisomal homo-octameric flavoprotein, was introduced into S. cerevisiae. Transformed cells contain varying amounts of alcohol oxidase depending on the plasmid used. Immunocytochemical experiments indicate that… CONTINUE READING

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