Import inhibition of poly(His) containing chloroplast precursor proteins by Ni2+ ions.

@article{Rothen1997ImportIO,
  title={Import inhibition of poly(His) containing chloroplast precursor proteins by Ni2+ ions.},
  author={Rudolf Rothen and M Thiess and Peter Schumann and Arminio Boschetti},
  journal={FEBS letters},
  year={1997},
  volume={403 1},
  pages={
          15-8
        }
}
The precursor of the small subunit of ribulose-1,5-bisphosphate carboxylase (pSS) and a modified pSS containing a C-terminal hexahistidyl tail (pSS(His)6) were imported into isolated Chlamydomonas chloroplasts with comparable efficiency. In the presence of Ni2+ ions the import of pSS(His)6 was inhibited and the precursor bound to the envelope remained protease sensitive, while import of pSS was not affected. Addition of an excess of L-histidine suppressed the inhibition demonstrating that the… CONTINUE READING