Import and processing of the precursor of the Rieske FeS protein of tobacco chloroplasts

@article{Madueo2004ImportAP,
  title={Import and processing of the precursor of the Rieske FeS protein of tobacco chloroplasts},
  author={Francisco Madue{\~n}o and Johnathan A. Napier and Francisco Javier Cejudo and J. C. Gray},
  journal={Plant Molecular Biology},
  year={2004},
  volume={20},
  pages={289-299}
}
AbstractcDNA clones encoding the precursor of the Rieske FeS protein of tobacco chloroplasts have been characterised and shown to derive from two different genes. The 5′ ends of the corresponding transcripts have been cloned using primer extension and PCR. The nucleotide sequences of the cDNAs (and their 5′ extensions) predict precursors for the tobacco proteins which differ in 4 amino acid residues out of a total of 228 residues and show high homology with the pea and spinach precursors. The… Expand
Import of the precursor of the chloroplast Rieske iron-sulphur protein by pea chloroplasts
TLDR
cDNA clones encoding the precursor of the Rieske FeS protein of the chloroplast thylakoid membrane have been isolated from a pea leaf cDNA library in λgt 11 and southern hybridisation to pea genomic DNA indicated the presence of a single gene encoding the Ryingke FeF protein in the haploid genome. Expand
Tissue-specific, light-regulated and plastid-regulated expression of the single-copy nuclear gene encoding the chloroplast Rieske FeS protein of Arabidopsis thaliana.
The single-copy PetC gene encoding the chloroplast Rieske FeS protein of Arabidopsis thaliana consists of five exons interrupted by four introns and encodes a protein of 229 amino acid residues withExpand
A potyvirus P1 protein interacts with the Rieske Fe/S protein of its host.
TLDR
Y2H assays showed that only the N-terminal part of SMV-P P1 was responsible for the interaction with the Rieske Fe/S protein and that amino acids 1-33 interacted only with the transit peptide, while amino acids 34-82 could interact with the entire Riesce Fe/ S protein. Expand
Iron-sulfur cluster reconstitution of spinach chloroplast Rieske protein requires a partially prefolded apoprotein.
TLDR
The data presented here are the first to demonstrate successful chemical reconstitution of the 2Fe-2S cluster into a Rieske apoprotein from higher plant chloroplasts, and suggest that upon cluster integration, further folding occurs, allowing the Rieskel protein to attain a final, native structure. Expand
Inducible Repression of Nuclear-Encoded Subunits of the Cytochrome b6f Complex in Tobacco Reveals an Extraordinarily Long Lifetime of the Complex1[W][OPEN]
TLDR
An ethanol-inducible RNA interference approach targeted against the essential nuclear-encoded Rieske protein (PetC) and the small M subunit (PetM) indicated a lifetime of the cytochrome b6f complex of at least 1 week. Expand
Conservative sorting in a primitive plastid
TLDR
The operation of the Sec and Tat pathways in cyanelles are demonstrated and for the first time in vitro protein import across cyanobacteria‐like thylakoid membranes and protease protection of the mature protein is shown. Expand
BcRISP1, isolated from non-heading Chinese cabbage, decreases the seed set of transgenic Arabidopsis
Mitochondria are the energy sources of plant cells and are involved in regulating cell development. Ubiquinol–cytochrome c reductase iron-sulfur protein, which is necessary for mitochondrialExpand
Multiple plastid signals regulate the expression of the pea plastocyanin gene in pea and transgenic tobacco plants.
  • J. Sullivan, J. Gray
  • Biology, Medicine
  • The Plant journal : for cell and molecular biology
  • 2002
TLDR
Evidence is provided for multiple plastid signals operating at different developmental stages and affecting transcriptional and post-transcriptional processes regulating expression of the pea PetE gene. Expand
Multiple Rieske proteins in prokaryotes: where and why?
TLDR
It is suggested that the existence of multiple high potential Rieske proteins in prokaryotes could be one way of allowing an organism to adapt their electron transfer chains to changing environmental conditions. Expand
Analysis of the chloroplast protein complexes by blue-native polyacrylamide gel electrophoresis (BN-PAGE)
TLDR
Analysis of chloroplast protein complexes by blue-native gel electrophoresis will allow the generation of 'protein maps' from different species, tissues and developmental stages or from mutant organelles. Expand
...
1
2
...

References

SHOWING 1-10 OF 45 REFERENCES
Import of the precursor of the chloroplast Rieske iron-sulphur protein by pea chloroplasts
TLDR
cDNA clones encoding the precursor of the Rieske FeS protein of the chloroplast thylakoid membrane have been isolated from a pea leaf cDNA library in λgt 11 and southern hybridisation to pea genomic DNA indicated the presence of a single gene encoding the Ryingke FeF protein in the haploid genome. Expand
The complete amino-acid sequence of the rieske FeS-precursor protein from spinach chloroplasts deduced from cDNA analysis
TLDR
Alignteins of the sequence with sequences from Rieske FeS-proteins of respiratory electron transport chains shows little sequence homology, but remarkable similarity in secondary structure including a putative N-terminal transmembrane segment of about 25 residues and the peptides CTHLGCV and CPCHGS in the C-terminals that are involved in the binding of the Fe2S2-cluster. Expand
Characterisation of a full-length cDNA clone for pea ferredoxin-NADP+ reductase
TLDR
Southern hybridisation to pea genomic DNA indicates that there are probably two genes in the haploid genome. Expand
The Transport of Proteins into Chloroplasts
TLDR
All the properties found for binding of proteins to isolated envelopes are consistent with the concept of the so-called envelope carrier hypothesis. Expand
Biosynthesis of the chloroplast cytochrome b6f complex: studies in a photosynthetic mutant of Lemna.
The biosynthesis of the cytochrome b6f complex has been studied in a mutant, no. 1073, of Lemna perpusilla that contained less than 1% of the four protein subunits when compared with a wild-typeExpand
Loss of efficient import and thylakoid insertion due to N- and C-terminal deletions in the light-harvesting chlorophyll a/b binding protein.
TLDR
Results demonstrate that there are two N-terminal sites that are cleaved during import of the LHCP precursor, undoubtedly contributing to the heterogeneity of LHCP found in vivo. Expand
A thylakoid processing protease is required for complete maturation of the lumen protein plastocyanin
TLDR
It is shown here that the stromal processing protease3 is not responsible for both processing events of plastocyanin import, and that a novel protease located in the thylakoids processes this intermediate to the mature protein. Expand
Isolation of cDNA clones for fourteen nuclear-encoded thylakoid membrane proteins
TLDR
Spinach cDNA libraries, made from polyadenylated seedling RNA, have been constructed in pBR322 and the expression vector λgt11 and contain components containing antigenic determinants against the lysine-rich 34 kd proteins. Expand
Transit peptides of nuclear‐encoded chloroplast proteins share a common amino acid framework.
TLDR
It is proposed that these three shared sequence elements form a common framework in transit‐bearing chloroplast precursors which mediates the common functions performed by each transit peptide. Expand
N‐terminal amino acid sequence of the Rieske iron‐sulfur protein from the cytochrome b 6/f‐complex of spinach thylakoids
The 20 kDa subunit of the cytochrome b 6/f‐complex, the Rieske iron‐sulfur protein, was isolated by preparative SDS‐PAGE. For automatic Edman degradation the protein was recovered by electrophoreticExpand
...
1
2
3
4
5
...