Implications of CRISPR-Based Germline Engineering for Cancer Survivors

@article{Katz2017ImplicationsOC,
  title={Implications of CRISPR-Based Germline Engineering for Cancer Survivors},
  author={Gregory Katz and Peter J. Pitts},
  journal={Therapeutic Innovation \& Regulatory Science},
  year={2017},
  volume={51},
  pages={672 - 682}
}
Cancer survivors can carry germline mutations that will be transmitted to their progeny. Today, many of these mutations have been identified and can be tracked. With the recent development of genome-editing technologies and CRISPR (clustered regularly interspaced short palindromic repeats), the possibility of genetically modifying the human germline—gametes and embryos—has never been closer. This perspective has sparked a controversy within the scientific community with reactions ranging from… Expand
CRISPR-Cas9
TLDR
This work delved into CRISPR-Cas9 from its natural and ortholog origins to its engineered variants and behaviors to present its notable and diverse applications in the fields of biotechnology and human therapeutics. Expand

References

SHOWING 1-10 OF 125 REFERENCES
Ethics and germline gene editing
TLDR
Although these experiments were performed in nonviable, triploid embryos that were neither intended nor suitable for clinical use, the work nonetheless demonstrates how the prospect of manipulating the human germline elicits hopes and fears and triggers moral debates. Expand
Genome engineering through CRISPR/Cas9 technology in the human germline and pluripotent stem cells.
TLDR
This narrative review seeks to understand the possible impact of CRISR/Cas9 technology on human reproduction from the technical and ethical point of view, and suggest a course of action for the scientific community. Expand
International regulatory landscape and integration of corrective genome editing into in vitro fertilization
TLDR
Current status of genome editing in mammalian embryonic stem cells and zygotes is examined and potential issues in the international regulatory landscape regarding human germline gene modification are discussed, addressing some ethical and social issues that would be raised when each country considers whether genome editing-mediated germ line gene correction for preventive medicine should be permitted. Expand
Safeguarding gene drive experiments in the laboratory
Multiple stringent confinement strategies should be used whenever possible Gene drive systems promote the spread of genetic elements through populations by assuring they are inherited more often thanExpand
Safeguarding CRISPR-Cas9 gene drives in yeast
TLDR
The efficacy of CRISPR-Cas9 gene drive systems in wild and laboratory strains of the yeast Saccharomyces cerevisiae is reported and concerns surrounding accidental genome editing are addressed by developing and validating methods of molecular confinement that minimize the risk of unwanted genome editing. Expand
Human Germline Interventions–Think First
  • E. Hildt
  • Computer Science, Medicine
  • Front. Genet.
  • 2016
TLDR
This work states that there is consensus that possible future uses in somatic cells hold enormous promise in clinical contexts, but human germline interventions, i.e., the genetic modification of human gametes or embryos, are highly controversial. Expand
RNA Interference in the Age of CRISPR: Will CRISPR Interfere with RNAi?
TLDR
RNAi will still occupy specific domains of biomedical research and clinical applications, under the current state of development of these technologies, however, further improvements in CRISPR/Cas9 based technology may ultimately enable it to dominate RNAi in the long term. Expand
Where in the world could the first CRISPR baby be born?
TLDR
Around the world, scientists are gathering to discuss the promise and perils of editing the genome of a human embryo, prompted by an explosion of interest in the powerful technology known as CRISPR/Cas9, which has brought unprecedented ease and precision to genetic engineering. Expand
Correction of a genetic disease in mouse via use of CRISPR-Cas9.
TLDR
It is shown that mice with a dominant mutation in Crygc gene that causes cataracts could be rescued by coinjection into zygotes of Cas9 mRNA and a single-guide RNA targeting the mutant allele, and were fertile and able to transmit the corrected allele to their progeny. Expand
Targeted Germline Modifications in Rats Using CRISPR/Cas9 and Spermatogonial Stem Cells.
TLDR
Spermatogonial gene editing with CRISPR/Cas9 provided a platform for generating targeted germline mutations in rats and for studying spermatogenesis. Expand
...
1
2
3
4
5
...