Implication of an Outer Surface Lipoprotein in Adhesion of Bifidobacterium bifidum to Caco-2 Cells

@article{Guglielmetti2008ImplicationOA,
  title={Implication of an Outer Surface Lipoprotein in Adhesion of Bifidobacterium bifidum to Caco-2 Cells},
  author={S. Guglielmetti and I. Tamagnini and D. Mora and M. Minuzzo and A. Scarafoni and S. Arioli and J. Hellman and M. Karp and C. Parini},
  journal={Applied and Environmental Microbiology},
  year={2008},
  volume={74},
  pages={4695 - 4702}
}
ABSTRACT We found that the human intestinal isolate Bifidobacterium bifidum MIMBb75 strongly adhered to Caco-2 cells. Proteinase K and lithium chloride treatments showed that proteins play a key role in MIMBb75 adhesion to Caco-2 cells. By studying the cell wall-associated proteins, we identified a surface protein, which we labeled BopA. We purified the protein chromatographically and found that it functioned as an adhesion promoter on Caco-2 cells. In silico analysis of the gene coding for… Expand
Study of the Adhesion of Bifidobacterium bifidum MIMBb75 to Human Intestinal Cell Lines
TLDR
It seems that the colonization strategy of this bacterium can be influenced by factors varying along the gastrointestinal tract, such as the presence of specific sugars and bile salts and the pH, possibly limiting the adhesion of B.bifidum to only restricted distal sites of the gut. Expand
BopA has no major role in the adhesion of Bifidobacterium bifidum to 1 intestinal epithelial cells , extracellular matrix proteins and mucus 2
28 The ability of bifidobacteria to adhere to the intestine of human host is considered to be 29 important for efficient colonization and achieving probiotic effects. Bifidobacterium 30 bifidumExpand
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