Implementation of vibrational phase contrast coherent anti-Stokes Raman scattering microscopy.

  title={Implementation of vibrational phase contrast coherent anti-Stokes Raman scattering microscopy.},
  author={Martin Jurna and Jennifer L. Herek and Herman Leonard Offerhaus},
  journal={Applied optics},
  volume={50 13},
Detection of molecules using vibrational resonances in the fingerprint region for narrowband coherent anti-Stokes Raman scattering (CARS) is challenging. The spectrum is highly congested resulting in a large background and a reduced specificity. Recently we introduced vibrational phase contrast CARS (VPC-CARS) microscopy as a technique capable of detecting both the amplitude and phase of the CARS signal, providing background-free images and high specificity. In this paper we present a new… Expand
3 Citations
Non-resonant background suppression by destructive interference in coherent anti-Stokes Raman scattering spectroscopy.
This work proposes a new approach that suppresses the non-resonant background contribution to the measured signal in CARS spectroscopy while simultaneously yielding high spectral resolution. Expand
Spektrale Modulationen in der Femtosekunden-Stimulierten Raman-Mikroskopie
Femtosecond stimulated Raman microscopy (FSRM) is an upcoming technique in vibrational microscopy that combines optical imaging with the chemical sensitivity of Raman scattering. It allows for theExpand


Vibrational phase contrast microscopy by use of coherent anti-Stokes Raman scattering.
In biological samples the resonant coherent anti-Stokes Raman scattering signal of less abundant constituents can be overwhelmed by the nonresonant background, preventing detection of thoseExpand
Visualizing resonances in the complex plane with vibrational phase contrast coherent anti-Stokes Raman scattering.
Using vibrational phase contrast CARS (VPC-CARS), enhanced sensitivity in quantitative measurements of ethanol/methanol mixtures and increased selectivity in a heterogeneous mixture of plastics and water is demonstrated. Expand
High-sensitivity vibrational imaging with frequency modulation coherent anti-Stokes Raman scattering (FM CARS) microscopy.
A new approach to coherent anti-Stokes Raman scattering (CARS) microscopy is demonstrated that significantly increases the detection sensitivity and allows for the detection of far fewer vibrational oscillators than possible through existing CARS microscopy methods. Expand
Heterodyne coherent anti-Stokes Raman scattering (CARS) imaging.
It is shown that heterodyne CARS microscopy permits the detection of weak vibrational resonances that are otherwise overshadowed by the strong interference of the nonresonant background. Expand
Time-resolved coherent anti-Stokes Raman scattering microscopy: Imaging based on Raman free induction decay
A time-resolved coherent anti-Stokes Raman scattering (CARS) microscope allows three-dimensional imaging based on Raman free induction decay of molecular vibration with no requirement for labeling ofExpand
Polarization coherent anti-Stokes Raman scattering microscopy.
P-CARS microscopy that allows vibrational imaging with high sensitivity and spectral selectivity and imaging of unstained cells based on the contrast of the protein amide I band is reported. Expand
Differential two-signal picosecond-pulse coherent anti-Stokes Raman scattering imaging microscopy by using a dual-mode optical parametric oscillator.
A novel differential two-signal technique of coherent anti-Stokes Raman scattering (CARS) imaging microscopy using a picosecond optical parametric oscillator (OPO) operated in two stable modes separated by a few nanometers is proposed. Expand
Background free CARS imaging by phase sensitive heterodyne CARS.
Heterodyne CARS, based on a controlled and stable phase-preserving chain, can be used to measure amplitude and phase information of molecular vibration modes and the resulting improvement of the signal to noise ratio is shown. Expand
Coherent anti-stokes Raman scattering microscopy: chemical imaging for biology and medicine.
  • C. Evans, X. Xie
  • Biology, Chemistry
  • Annual review of analytical chemistry
  • 2008
Coherent anti-Stokes Raman scattering (CARS) microscopy is a label-free imaging technique that is capable of real-time, nonperturbative examination of living cells and organisms based on molecularExpand
Characterization of photodamage in coherent anti-Stokes Raman scattering microscopy.
This study suggests that for dense samples such as the myelin sheath, CARS imaging induced photodamage can be minimized by using laser beams with relatively long near IR wavelengths and a repetition rate of a few MHz and for less dense samples, laser pulses of higher repetition rates are preferred. Expand