Implementation of the CRISPR-Cas9 system in fission yeast

  title={Implementation of the CRISPR-Cas9 system in fission yeast},
  author={Jake Z. Jacobs and Keith M. Ciccaglione and Vincent Tournier and Mikel Zaratiegui},
  booktitle={Nature communications},
Application of the CRISPR-Cas9 genome editing system in the model organism Schizosaccharomyces pombe has been hampered by the lack of constructs to express RNA of arbitrary sequence. Here we present expression constructs that use the promoter/leader RNA of K RNA (rrk1) and a ribozyme to produce the targeting guide RNA. Together with constitutive expression of Cas9, this system achieves selection-free specific mutagenesis with efficiencies approaching 100%. The rrk1 CRISPR-Cas9 method enables… CONTINUE READING
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