Implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry.

@article{Eyigor2002ImplementationOR,
  title={Implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry.},
  author={Aysegul Eyigor and Kamil Tayfun Carli and Cetin Unal},
  journal={Letters in applied microbiology},
  year={2002},
  volume={34 1},
  pages={
          37-41
        }
}
AIMS The present study describes the implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry. METHODS AND RESULTS Real-time PCR with Salmonella invA-specific primers and a standard bacteriological method was applied to detect Salmonella in tetrathionate enrichment cultures of 492 intestinal homogenates and 27 drag swabs from 47 poultry flocks. The number of positive individual samples by real-time PCR and culture method was 65 (12.5%) and 35… CONTINUE READING
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