Implementation of a one-step real-time RT-PCR protocol for diagnosis of foot-and-mouth disease.

@article{Shaw2007ImplementationOA,
  title={Implementation of a one-step real-time RT-PCR protocol for diagnosis of foot-and-mouth disease.},
  author={Andrew E. Shaw and Scott M. Reid and Katja Ebert and Geoffrey H. Hutchings and Nigel P. Ferris and Donald P. King},
  journal={Journal of virological methods},
  year={2007},
  volume={143 1},
  pages={81-5}
}
An automated one-step real-time reverse transcription polymerase chain reaction (rRT-PCR) protocol was optimised and evaluated for the routine diagnosis of foot-and-mouth disease (FMD). Parallel testing of RNA samples (n=257) indicated that this assay has a diagnostic sensitivity at least equivalent to the automated two-step rRT-PCR protocol previously used for the laboratory detection of FMD virus (FMDV). This more rapid and economical one-step protocol will play a key role in contingency… CONTINUE READING

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