Imperfection and radiation damage in protein crystals studied with coherent radiation

  title={Imperfection and radiation damage in protein crystals studied with coherent radiation},
  author={C. Nave and G. Sutton and G. Evans and R. Owen and C. Rau and Ian Robinson and D. Stuart},
  journal={Journal of Synchrotron Radiation},
  pages={228 - 237}
Coherent diffraction observations from polyhedra crystals at cryotemperature are reported. Information is obtained about the lattice strain and the changes with radiation damage. 
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Matching X-ray beam and detector properties to protein crystals of different perfection
  • C. Nave
  • Physics, Medicine
  • Journal of synchrotron radiation
  • 2014
Expressions are given to match X-ray data collection facilities to the intrinsic diffraction properties of crystals with different degrees of perfection.
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X-ray topography methods were employed to identify defects in lysozyme crystals. White-beam and monochromatic topographs of lysozyme crystals obtained at the National Synchrotron Light Source areExpand
Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging
A combination of techniques, including Bragg coherent diffractive imaging, are employed to characterise the radiation induced damage in a micron-sized protein crystal over time and could help screen for potential protein crystal candidates for measurement at X-ray free election laser sources. Expand
The minimum crystal size needed for a complete diffraction data set
  • J. Holton, K. Frankel
  • Chemistry, Medicine
  • Acta crystallographica. Section D, Biological crystallography
  • 2010
A formula for absolute scattering power is derived to include spot fading arising from radiation damage and the crystal volume needed to collect diffraction data to a given resolution is calculated.
Coherent X-ray diffractive imaging of protein crystals.
Qualitative analysis of a number of single-crystal diffraction peaks indicates the presence of inward surface contraction on 2 micrometer-size protein crystals, and a plausible model is constructed. Expand
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Different types of crystal twinning are reviewed with an emphasis on how to detect the phenomenon from protein diffraction data. The recent literature and a database survey both serve as reminders toExpand
Continuous X-ray diffractive field in protein nanocrystallography.
This paper demonstrates an approach that is specifically tailored to nanocrystalline samples and offers a unique crystallographic solution that can treat proteins under conditions close to the active form. Expand
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A description of imperfections in protein crystals.
  • C. Nave
  • Materials Science, Medicine
  • Acta crystallographica. Section D, Biological crystallography
  • 1998
An analysis is given of the contribution of various crystal imperfections to the rocking widths of reflections and the divergence of the diffracted beams that has implications for improving crystal perfection, defining data-collection requirements and for data-processing procedures. Expand