Imparting Bone Affinity to Glycoproteins Through the Conjugation of Bisphosphonates

  title={Imparting Bone Affinity to Glycoproteins Through the Conjugation of Bisphosphonates},
  author={S{\'e}bastien A. Gittens and John R Matyas and Ronald F. Zernicke and Hasan Uludağ},
  journal={Pharmaceutical Research},
Purpose. To develop a novel means of conjugating bisphosphonates onto the carbohydrate moieties of glycoproteins to enhance protein affinity to bone. Methods. 1-Amino-1,1-diphosphonate methane (aminoBP) was conjugated onto the carbohydrate moietites of oxidized fetuin by using 4-(maleimidomethyl)cyclohexane-1-carboxyl-hydrazide (MMCCH). Bone affinity of the resulting conjugates was compared to proteins obtained from another means of conjugation, whereby aminoBP was conjugated onto fetuin's… CONTINUE READING


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