Impairment of calcineurin function in Neurospora crassa reveals its essential role in hyphal growth, morphology and maintenance of the apical Ca2+ gradient

@article{Prokisch1997ImpairmentOC,
  title={Impairment of calcineurin function in Neurospora crassa reveals its essential role in hyphal growth, morphology and maintenance of the apical Ca2+ gradient},
  author={Holger Prokisch and Oded Yarden and M. Dieminger and M. Tropschug and Ilse Babette Barthelmess},
  journal={Molecular and General Genetics MGG},
  year={1997},
  volume={256},
  pages={104-114}
}
The function of Neurospora crassa calcineurin was investigated in N. crassa strains transformed with a construct that provides for the inducible expression of antisense RNA for the catalytic subunit of calcineurin (cna-1). Induction of antisense RNA expression was associated with reduced levels of cna-1 mRNA and of immunodetectable CNA1 protein and decreased calcineurin enzyme activity, indicating that a conditional reduction of the target function had been achieved in antisense transformants… CONTINUE READING