Impaired splicing of the rps 12 transcript in ribosome-deficient plastids

  title={Impaired splicing of the rps 12 transcript in ribosome-deficient plastids},
  author={Thomas H{\"u}bschmann and Wolfgang R. Hess and Thomas B{\"o}rner},
  journal={Plant Molecular Biology},
Analysis of RNA maturation in ribosome-deficient plastids of four non-allelic barley mutants revealed an increased accumulation and altered processing of transcripts of the ribosomal protein gene CS12 (rps12) compared to normal chloroplasts. The three exons of rps12 are part of two different polycistronic transcription units. Generation of mature rps12-mRNA involves both cis-and trans-splicing. In ribosome-deficient plastids, the cis-intron separating exons 2 and 3 remains entirely unspliced… CONTINUE READING

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