Host defense against infection by human cytomegalovirus (HCMV) is ensured in great part by cytotoxic CD8(+) T lymphocytes (CTLs) directed against the tegument protein pp65. The hyperimmediate release of incoming pp65 into the major histocompatibility complex (MHC) class I pathway after fusion of the virus with the cell membrane provides a very early… (More)
FIGURE 3. RPE cells were able to present endogenously derived pp65 peptides to CTLs. HLA-A2 positive U373MG and RPE cells were either pulsed overnight with the HLA-A2–binding peptide N9V or irrelevant peptide I9Y at 500 nM or infected with pp65 recombinant adenoviruses Ad-pp65s and Ad-pp65as, sense and antisense, respectively. Cells were used as targets in a 51Cr-release assay at different E-T ratios.