Impaired ex vivo lipopolysaccharide-stimulated whole blood tumor necrosis factor production may identify "septic" intensive care unit patients.

@article{Heagy2000ImpairedEV,
  title={Impaired ex vivo lipopolysaccharide-stimulated whole blood tumor necrosis factor production may identify "septic" intensive care unit patients.},
  author={Wyrta Heagy and Christopher F. Hansen and Kimberly M Nieman and Marilyn Cohen and C. E. Richardson and Jorge Arturo de Le{\'o}n Rodriguez and Michael A. West},
  journal={Shock},
  year={2000},
  volume={14 3},
  pages={271-6; discussion 276-7}
}
Currently, there is no reliable diagnostic test to identify septic intensive care unit (ICU) patients. We initiated studies to test the hypothesis that in sepsis, the in vivo exposure to endotoxin is detectable by the ex vivo analysis of lipopolysaccharide (LPS)-stimulated tumor necrosis factor (TNF) production. We obtained heparinized whole blood (WB) from 58 ICU patients and 14 healthy controls. The samples were incubated +/-10 ng/mL of LPS at 37 degrees C for 3 h. Plasma TNF levels were… CONTINUE READING

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