Impaired cytoskeletal organization and membrane integrity in lens fibers of a Rho GTPase functional knockout transgenic mouse

@article{Maddala2004ImpairedCO,
  title={Impaired cytoskeletal organization and membrane integrity in lens fibers of a Rho GTPase functional knockout transgenic mouse},
  author={Rupalatha Maddala and P Deng and Joseph M Costello and Eric Wawrousek and Jacob S Zigler and V. Purnachandra Rao},
  journal={Laboratory Investigation},
  year={2004},
  volume={84},
  pages={679-692}
}
To investigate the effects of Rho GTPase inactivation on lens fiber cell cytoskeletal and morphological integrity, a transgenic mouse model expressing C3-exoenzyme (a bacterial toxin) in a lens-specific manner was utilized. Cryosections of whole eyes from C3 transgenic mice and littermate controls were stained for F-actin with rhodamine–phalloidin or immunostained for β-catenin, aquaporin-0 or connexin-50, and confocal images were recorded. Lens fiber cell morphology was examined at both light… CONTINUE READING
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Lens epithelial cell differentiation

  • AS Menko
  • Exp Eye Res
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