Impact of pore structural parameters on column performance and resolution of reversed-phase monolithic silica columns for peptides and proteins.


In this work, monolithic silica columns with the C4, C8, and C18 chemistry and having various macropore diameters and two different mesopore diameters are studied to access the differences in the column efficiency under isocratic elution conditions and the resolution of selected peptide pairs under reversed-phase gradient elution conditions for the… (More)


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