Impact of p53 knockout and topotecan treatment on gene expression profiles in human colon carcinoma cells: a pharmacogenomic study.

@article{Daoud2003ImpactOP,
  title={Impact of p53 knockout and topotecan treatment on gene expression profiles in human colon carcinoma cells: a pharmacogenomic study.},
  author={Sayed Salin Daoud and Peter J. Munson and William Reinhold and Lynn Marie Young and Vinay V. Prabhu and Qiang Yu and Jihyun LaRose and Kurt W. Kohn and John N. Weinstein and Yves Pommier},
  journal={Cancer research},
  year={2003},
  volume={63 11},
  pages={
          2782-93
        }
}
To uncover transcriptional stress responses related to p53, we used cDNA microarrays (National Cancer Institute Oncochips comprising 6500 different genes) to characterize the gene expression profiles of wild-type p53 HCT-116 cells and an isogenic p53 knockout counterpart after treatment with topotecan, a specific topoisomerase I inhibitor. The use of the p53 knockout cells had the advantage over p53-overexpressing systems in that p53 activation is mediated physiologically. RNA was extracted… CONTINUE READING

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References

Publications referenced by this paper.
SHOWING 1-10 OF 33 REFERENCES