Impact of methanol concentration on secreted protein production in oxygen-limited cultures of recombinant Pichia pastoris.

@article{Khatri2006ImpactOM,
  title={Impact of methanol concentration on secreted protein production in oxygen-limited cultures of recombinant Pichia pastoris.},
  author={Narendar K Khatri and Frank Hoffmann},
  journal={Biotechnology and bioengineering},
  year={2006},
  volume={93 5},
  pages={
          871-9
        }
}
The methylotrophic yeast Pichia pastoris is a powerful system for production of recombinant proteins, showing high ability to secrete properly folded proteins. A major plus is the strong AOX1 promoter highly induced by methanol. During growth on methanol, however, oxygen readily becomes limiting. In oxygen-limited cultivations of recombinant Pichia pastoris, the methanol concentration had a strong impact on the production of a single-chain antibody fragment (scFv). High methanol concentrations… CONTINUE READING
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