Impact of gene vector design on the control of recombinant monoclonal antibody production by chinese hamster ovary cells

@article{Davies2011ImpactOG,
  title={Impact of gene vector design on the control of recombinant monoclonal antibody production by chinese hamster ovary cells},
  author={Sarah L. Davies and Peter M. O'Callaghan and Jane McLeod and Leon P Pybus and Yun Hee Sung and James Rance and Stephen J. Wilkinson and Andrew J Racher and Robert J. Young and David C James},
  journal={Biotechnology Progress},
  year={2011},
  volume={27}
}
In this study, we systematically compare two vector design strategies for recombinant monoclonal antibody (Mab) synthesis by Chinese hamster ovary (CHO) cells; a dual open reading frame (ORF) expression vector utilizing separate cytomegalovirus (CMV) promoters to drive heavy chain (HC) and light chain (LC) expression independently, and a single ORF vector design employing a single CMV promoter to drive HC and LC polypeptide expression joined by a foot and mouth disease virus F2A polypeptide… 
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The Augmenting Effects of the tDNA Insulator on Stable Expression of Monoclonal Antibody in Chinese Hamster Ovary Cells.
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