Impact of blood volume, tube shaking, and incubation time on reproducibility of QuantiFERON-TB gold in-tube assay.

@article{Gaur2013ImpactOB,
  title={Impact of blood volume, tube shaking, and incubation time on reproducibility of QuantiFERON-TB gold in-tube assay.},
  author={Rajiv L Gaur and Madhukar Pai and Niaz Banaei},
  journal={Journal of clinical microbiology},
  year={2013},
  volume={51 11},
  pages={
          3521-6
        }
}
Gamma interferon (IFN-γ) release assays (IGRAs) are functional assays used serially to measure the efficacy of novel tuberculosis (TB) vaccines and to screen health care workers for latent tuberculosis infection (LTBI). However, studies have shown nonreproducible IGRA results. In this study, we investigated the effects of blood volume (0.8, 1.0, and 1.2 ml), tube shaking (gentle versus vigorous), and incubation duration (16, 20, and 24 h) on the reproducibility of QuantiFERON-TB Gold In-Tube… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 21 CITATIONS

Interferon Gamma Release Assays for Latent Tuberculosis: What Are the Sources of Variability?

VIEW 3 EXCERPTS
CITES METHODS & BACKGROUND

Reproducibility of interferon gamma (IFN-γ) release Assays. A systematic review.

VIEW 4 EXCERPTS
CITES BACKGROUND & RESULTS

The QuantiFERON-TB Gold In-Tube Assay in Neuro-Ophthalmology

VIEW 1 EXCERPT
CITES BACKGROUND

References

Publications referenced by this paper.
SHOWING 1-10 OF 26 REFERENCES

Test variability of the QuantiFERON-TB gold in-tube assay in clinical practice.

VIEW 1 EXCERPT