Impact of antifreeze proteins and antifreeze glycoproteins on bovine sperm during freeze-thaw.

@article{Prathalingam2006ImpactOA,
  title={Impact of antifreeze proteins and antifreeze glycoproteins on bovine sperm during freeze-thaw.},
  author={Nilendran S Prathalingam and William Vincent Holt and Stuart G Revell and Samantha M. Mirczuk and Roland A. Fleck and Paul F Watson},
  journal={Theriogenology},
  year={2006},
  volume={66 8},
  pages={1894-900}
}
There are no reports on the use of antifreeze proteins (AFP) and antifreeze glycoproteins (AFGP) for the use of bull sperm cryopreservation despite studies in the ram, mouse and chimpanzee. The effect of freezing and thawing on bull sperm viability, osmotic resistance and acrosome integrity were observed following the addition of AFP1, AFPIII and AFGP at four concentrations (0.1, 1, 10 and 100 microg/ml). In a second part of the experiment, fluorescein was conjugated to the AFPs and AFGP and… CONTINUE READING