Impact of a basement membrane-degrading protease on dissemination and secondary infection of Autographa californica multiple nucleopolyhedrovirus in Heliothis virescens (Fabricus).

@article{Li2007ImpactOA,
  title={Impact of a basement membrane-degrading protease on dissemination and secondary infection of Autographa californica multiple nucleopolyhedrovirus in Heliothis virescens (Fabricus).},
  author={Hua-rong Li and Hailin Tang and Robert L. Harrison and Bryony C. Bonning},
  journal={The Journal of general virology},
  year={2007},
  volume={88 Pt 4},
  pages={
          1109-19
        }
}
ScathL is a cathepsin L-like cysteine protease from the flesh fly, Sarcophaga peregrina, that digests components of the basement membrane (BM) during insect metamorphosis. A recombinant baculovirus that expresses ScathL (AcMLF9.ScathL) kills larvae of the tobacco budworm, Heliothis virescens, significantly faster than the wild-type virus and triggers melanization and tissue fragmentation in infected larvae shortly before death. As BMs are a potential barrier to the spread of baculovirus… Expand
Insecticidal activity of a basement membrane-degrading protease against Heliothis virescens (Fabricius) and Acyrthosiphon pisum (Harris).
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References

SHOWING 1-10 OF 42 REFERENCES
Use of Proteases to Improve the Insecticidal Activity of Baculoviruses
TLDR
Six recombinant clones of Autographa californica nucleopolyhedrovirus are constructed that express three proteases that digest basement membrane proteins: rat stromelysin-1, human gelatinase A, and flesh fly cathepsin L to highlight the potential of improving baculovirus efficacy through the expression of proteases. Expand
Central Role of Hemocytes in Autographa californica M Nucleopolyhedrovirus Pathogenesis in Heliothis virescens and Helicoverpa zea
TLDR
Results demonstrated that hemocytes—by removing virus from the hemolymph instead of amplifying it and by participating in the encapsulation of infection foci—together with the host's melanization response, formed the basis of H. zea's resistance to fatal infection by AcMNPV. Expand
Co-infection of Manduca sexta larvae with polydnavirus from Cotesia congregata increases susceptibility to fatal infection by Autographa californica M Nucleopolyhedrovirus.
TLDR
The results showed that during the first 9 days after inoculation, parasitized and polydnavirus-inoculated M. sexta larvae died more quickly and at higher levels than nonparasitized and sham-injected controls, suggesting that the cellular immune response was a factor in conferring resistance to fatal infection by AcMNPV-hsp70/lacZ. Expand
Comparative pathogenesis of Autographa californica M nuclear polyhedrosis virus in larvae of Trichoplusia ni and Heliothis virescens.
TLDR
Dose-mortality relationships suggested that the peritrophic membrane provided little protection from AcMNPV infections for both Trichoplusia ni and Heliothis virescens. Expand
Use of Scorpion Neurotoxins to Improve the Insecticidal Activity of Rachiplusia ou Multicapsid Nucleopolyhedrovirus
TLDR
RoMNPV recombinant virus expressing AaIT from the p10 promoter produced a reduced quantity of viral occlusions and polyhedrin protein and failed to occlude virions efficiently, but no significant difference in speed of kill was observed between Aa IT- and LqhIT2-expressing viruses in this host. Expand
In vivo pathway of Autographa californica baculovirus invasion and infection.
TLDR
It is proposed that this budded virus, possessing an envelope with a peplomer structure, is the primary inoculum for the systemic invasion of the insect host. Expand
The insect tracheal system: a conduit for the systemic spread of Autographa californica M nuclear polyhedrosis virus.
TLDR
It is discovered that infection of the larval insect tracheal system (and not hemocytes, as thought previously) provides the major conduit for this virus to pass through basal laminae and to spread throughout the host. Expand
Infection of silkmoth follicular cells with Bombyx mori nuclear polyhedrosis virus
TLDR
Injection of collagenase into the pupae prior to viral injection was found to be effective in partially removing the basement membrane from the follicular epithelium and increasing the rate of viral spread throughout the tissue. Expand
The pathway of infection of Autographa californica nuclear polyhedrosis virus in an insect host.
An immunohistochemical study was conducted to detect the temporal infection sequence of Autographa californica M nuclear polyhedrosis virus in Trichoplusia ni larvae. Staining patterns indicated thatExpand
Pathogenesis of Rift Valley fever virus in mosquitoes--tracheal conduits & the basal lamina as an extra-cellular barrier.
TLDR
Evidence indicates the existence of a salivary gland infection barrier in Anopheles stephensi and the basal lamina may prevent access to mosquito cell surface virus receptors and help explain why anopheline mosquitoes are relatively incompetent arbovirus transmitters when compared to culicines. Expand
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