Background: Ventilator-associated pneumonia (VAP) is considered a serious health hazard among ventilated patients. The frequency of VAP in the ICUs is high and its negative impact on patient outcomes and resource utilization is huge. Aim of the Study: This study was carried out in order to examine the effect of a structured teaching program for prevention of ventilator-associated pneumonia on knowledge and practices of intensive care nurses at Central Quwesna Hospital, in Egypt. Design: A quasi-experimental research design was utilized. Setting: This study was conducted at the Central Quwesna Hospital in the intensive care unit. Subjects: Purposive sample consisted of all nurses, (30 nurses) who provide care for patients admitted to the intensive care unit in the previously mentioned hospital. Tools: Pre/posttest interview questionnaire sheet: It was designed to test nurse's knowledge about prevention of ventilator associated pneumonia, an observational checklist (pre/posttest), and teaching program for prevention of VAP. Results: The study findings revealed that, 30% of respondents were in the age group of 21-25 years followed by 36.7% in the age group of 26-30 years and 33.3% in the age group of above 30 years with a mean age of 32.32 years. Also, in relation to gender, 76.7% of nurses were found to be females. Concerning total clinical experience 43.3% of the nurses had 1-5 years of clinical experience, 30% had 6-10 years of clinical experience and 26.7% had above 10 years of clinical experience. With respect to professional educational qualification, it was observed that 23.3% of the respondents were having bachelor degree in nursing and 76.7% of respondents had technical institute degree in Nursing. As well, it was cleared that none of the nurses in the study sample had satisfactory total knowledge score at the pre-program implementation, however, the score of total knowledge increased immediately after the program, and continued to be higher at the first follow-up phase. Also, none of the nurses in the studied sample Correspondence to: Dr. Amina I. Badawy E-mail: email@example.com adequate practice at the pre-program phase, however, the score of total practice increased immediately after the program, and continued to be higher at the first follow-up phase. Moreover, a strong positive correlation was found between age, experience, knowledge, and practices of study subjects. As well, knowledge and practices were positively correlated. In addition, sex, education, and years of experience as sociodemographic variables were having an impact on knowledge, and practices regarding prevention of VAP among study subjects. Conclusion: The current study shows that nurses were having unsatisfactory total knowledge and practice at the preprogram implementation, however, the score of total knowledge increased immediately after the program, and continued to be higher at the first follow-up phase, indicating that the nurses gained knowledge after implementation of teaching program. On the other hand it was observed that there were association between age, years of experience, knowledge, and practice of intensive care nurses on prevention of VAP. As well, sex, level of education, and years of experience were found to have an impact on knowledge and practice scores. Recommendations: The current study recommended developing manuals, information booklets and self-instruction module in areas of prevention of VAP.