Impact of Laparoscopic CO2-insufflation on Tumor-associated molecules in cultured colorectal cancer cells

Abstract

Background: Laparoscopic CO2-insufflation is believed to stimulate proliferation and metastatic potential of gastrointestinal carcinomas. E-cadherin, I-CAM1, I-CAM2, and CD44 are involved into the metastatic process of different cancer cell lines. The current study investigates the influence of CO2-insufflation on the expression of E-cadherin, I-CAM1, I-CAM2, and CD44 in vitro. Methods: CX-2 and CC531 colon carcinoma cells (human/rat) were exposed to pneumoperitoneal CO2-insufflation. E-cadherin, I-CAM1, I-CAM2, and CD44 were measured 0, 12, 24, 48, and 72 h after CO2-insufflation using flowcytometry. Control groups were expo-sed to room air. Data were analyzed by the Wilcoxon–Mann–Whitney U-test. Results: Both cell lines showed significant alteration in E-cadherin, I-CAM1, and CD44 expression after CO2 exposure (p <0.05). No significant differences were found regarding I-CAM2 expression. Conclusion: The present study demonstrates CO2-insufflation to influence the expression of E-cadherin, I-CAM1 and CD44. Whether these changes increases the metastatic potential of colorectal cancer cells in vivo needs further investigation.

DOI: 10.1007/s00464-001-9194-3

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@article{Kim2001ImpactOL, title={Impact of Laparoscopic CO2-insufflation on Tumor-associated molecules in cultured colorectal cancer cells}, author={Z. G. Kim and C. Mehl and M. Lorenz and C. N. Gutt}, journal={Surgical Endoscopy And Other Interventional Techniques}, year={2001}, volume={16}, pages={1182-1186} }