Impact of Freeze-thaw Cycles and Storage Time on Plasma Samples Used in Mass Spectrometry Based Biomarker Discovery Projects

@article{Mitchell2005ImpactOF,
  title={Impact of Freeze-thaw Cycles and Storage Time on Plasma Samples Used in Mass Spectrometry Based Biomarker Discovery Projects},
  author={Breeana L Mitchell and Yutaka Yasui and Christopher I. Li and Annette L. Fitzpatrick and Paul D Lampe},
  journal={Cancer Informatics},
  year={2005},
  volume={1},
  pages={98 - 104}
}
Mass spectrometry approaches to biomarker discovery in human fluids have received a great deal of attention in recent years. While mass spectrometry instrumentation and analysis approaches have been widely investigated, little attention has been paid to how sample handling can impact the plasma proteome and therefore influence biomarker discovery. We have investigated the effects of two main aspects of sample handling on MALDI-TOF data: repeated freeze-thaw cycles and the effects of long-term… CONTINUE READING