Impact of Aspergillus oryzae genomics on industrial production of metabolites

  title={Impact of Aspergillus oryzae genomics on industrial production of metabolites},
  author={Keietsu Abe and Katusya Gomi and Fumihiko Hasegawa and Masayuki Machida},
Aspergillus oryzae is used extensively for the production of the traditional Japanese fermented foods sake (rice wine), shoyu (soy sauce), and miso (soybean paste). In recent years, recombinant DNA technology has been used to enhance industrial enzyme production by A. oryzae. Recently completed genomic studies using expressed sequence tag (EST) analyses and whole-genome sequencing are quickly expanding the industrial potential of the fungus in biotechnology. Genes that have been newly… 
Bioconversion of soy under the influence of Aspergillus oryzae strains producing hydrolytic enzymes
Fermenting soy materials with the RCAM 01133 strain of A. oryzae is an alternative way to produce food ingredients with good sensory properties containing carbohydrates and biologically complete protein in easily digestible forms.
Effect of Novel Engineered Aspergillus oryzae on Soybean Meal Fermentation and Optimization of Cultivation Conditions
The filamentous fungus Aspergillus oryzae is an important strain in fermentation industry. In this study, to test the possibilities of improving polypeptides production, the soybean meal fermentation
Improvement of Free Fatty Acid Secretory Productivity in Aspergillus oryzae by Comprehensive Analysis on Time-Series Gene Expression
This study found that the transport and metabolism of inorganic ions are crucial in the initial stages of FFA production and revealed 16 candidate genes to be modified in conjunction with the faaA disruption and provided guidance for further gene modification for FFA biosynthesis in A. oryzae.
Genomics of Aspergillus oryzae: Learning from the History of Koji Mold and Exploration of Its Future
  • M. Machida, O. Yamada, K. Gomi
  • Biology
    DNA research : an international journal for rapid publication of reports on genes and genomes
  • 2008
The genome comparison and transcriptome analysis uncovered mechanisms for effective degradation of raw materials in SSC and found the temperature reduction process empirically adopted in the traditional soy-sauce fermentation processes has been found to be important to keep strong expression of the A. oryzae-specific extracellular hydrolases.
Genetic and Metabolic Engineering in Filamentous Fungi
This review summarises the variety of omic tools that have recently become established for filamentous fungi, discusses related challenges and perspectives and highlights genetic and metabolic engineering methods to rationally design metabolic fluxes in filamentous fungus.
[Genomics and metabolic engineering of filamentous fungi in the post-genomics era].
This review summarizes the recent development ingenomics, comparative genomics, transcriptomics, proteomics and metabolomics of filamentous fungi, and describes their applications and functions in reconstruction of metabolic network, discovery of novel proteins and genes, investigation of cell physiological and biochemical reactions, and strain breeding.
Prebiotic isomalto-oligosaccharide production from economic crops of Thailand
The production of Isomalto-oligosaccharide by fungal fermentation using economic crops such as rice and cassava as substrates was investigated and results show that the fermentation of rice with A. oryzae TISTR 3102 produced the highest concentrations of total reducing sugar and free amino nitrogen.
Beneficial effects of Aspergillus-derived protease preparations on colonic luminal environment
The effects of Aspergillus-derived protease preparations on the colonic luminal environment in rats is investigated to know whether dietary of these enzymes exert potentially beneficial effects on health in mammals.


High Level Expression of Recombinant Genes in Aspergillus Oryzae
The amounts of heterologous protein obtained make this system attractive for even moderately priced industrial enzymes, as an example, the Rhizomucor miehei derived aspartic proteinase was secreted with yields in excess of three grams per liter.
Safety evaluation of tannase enzyme preparation derived from Aspergillus oryzae.
Absence of aflatoxin biosynthesis in koji mold (Aspergillus sojae)
It is suggested that defects in aflR expression cause the lack of expression of aflatoxin-related genes which results in the absence of a flatoxin biosynthesis in A. sojae strains.
Genome sequencing and analysis of Aspergillus oryzae
Specific expansion of genes for secretory hydrolytic enzymes, amino acid metabolism and amino acid/sugar uptake transporters supports the idea that A. oryzae is an ideal microorganism for fermentation.
Cellulolytic Enzyme Production by Three Fungi Grown in a Ground Corn Cob Medium 1.
Strains NRRL 11236, ATCC 32630, and ATCC 9095 produced no detectable toxic substance, however, when an oil extract was made of fermented corn cobs, strain ATCC 32098 produced a significant kill of 23.1% of the embryos.
Transcriptional analysis of genes for energy catabolism and hydrolytic enzymes in the filamentous fungus Aspergillus oryzae using cDNA microarrays and expressed sequence tags
The wheat-bran culture gave the richest gene expression profile of hydrolytic enzymes and the lowest expression levels of catabolic genes (EMP, TCA) among the three media tested, suggesting that A. oryzae uses both EMP and TCA for glucose metabolism under AC conditions.
LaeA, a Regulator of Secondary Metabolism in Aspergillus spp
A novel Aspergillus nuclear protein, LaeA, is presented here as a global regulator of secondary metabolism in this genus, and strains show little difference in spore production compared to the wild type, indicating that the primary role of laeA is to regulate metabolic gene clusters.
Aspergillus flavus expressed sequence tags for identification of genes with putative roles in aflatoxin contamination of crops.
Genes potentially involved in the aflatoxin contamination process were identified in the ESTs sequenced, including the a Flatoxin biosynthetic pathway, signal transduction, global regulation, pathogenicity of the fungus, and stress response.
Homologs of Aflatoxin Biosynthesis Genes and Sequence of aflR in Aspergillus oryzae andAspergillus sojae
ABSTRACT The presence, but not expression, of homologs of three structural genes and a regulatory gene necessary for aflatoxin biosynthesis inAspergillus parasiticus and A. flavus was shown for A.
Production of Biologically Active Recombinant Human Lactoferrin in Aspergillus Oryzae
The production of recombinant human lactoferrin in Aspergillus oryzae appears suitable for the large–scale production and secretion of biologically active mammalian glycoproteins.