Impact of 24-h cooling prior to freezing on the survival of domestic cat (Felis catus) epididymal sperm.

@article{Martins2009ImpactO2,
  title={Impact of 24-h cooling prior to freezing on the survival of domestic cat (Felis catus) epididymal sperm.},
  author={Jorge Luis Ara{\'u}jo Martins and Ana Izabel Silva Balbin Villaverde and Alana Fontales Lima and Paulo V M Steagall and J. C. P. Ferreira and Cesar Augusto Taconeli and Maria Denise Lopes},
  journal={Reproduction in domestic animals = Zuchthygiene},
  year={2009},
  volume={44 Suppl 2},
  pages={366-8}
}
The aim of this study was to investigate the impact of a 24-h cooling period prior to freezing on domestic cat epididymal sperm viability. Fifteen tomcats were submitted to routine orchiectomy and sperm samples were retrieved from both epididymides in a Tris-glucose-20% egg yolk extender. For each tomcat, the diluted sperm was split into two equal volumes and cooled to 5 degrees C at a rate of 0.5 degrees C/min; one sample for 60 min (control) and the other for 24 h (cooled). After the cooling… CONTINUE READING

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