Immunoturbidimetric measurement of serum retinol-binding protein in renal and hepatic disease.


Serum retinol-binding protein (RBP), with a biological half-life of less than 12 h, is a useful indicator of liver or kidney dysfunction. An automated immunoturbidimetric assay for the measurement of RBP has been developed using the Cobas-BIO centrifugal analyser and commercially available materials. Serum samples with RBP concentrations as low as 3 mg/L were measured. Within-run and day-to-day imprecision were 3.7 and 5.7%, respectively. The reference range (mean +/- 2SD) for 51 adults was 17 to 61 mg/L. Slight haemolysis of serum (haemoglobin 10 g/L) resulted in an apparent 8% reduction of RBP with greater interference at higher haemoglobin concentrations. However, bilirubin in concentrations up to 0.15 g/L did not interfere with RBP measurements. There was good correlation between immunoturbidimetry and a commercial radial immunodiffusion method. Serum RBP concentrations were decreased in liver disease and increased in renal failure.


Citations per Year

204 Citations

Semantic Scholar estimates that this publication has 204 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Bankson1988ImmunoturbidimetricMO, title={Immunoturbidimetric measurement of serum retinol-binding protein in renal and hepatic disease.}, author={Daniel D. Bankson and Nader Rifai and Lawrence M. Silverman}, journal={Annals of clinical biochemistry}, year={1988}, volume={25 ( Pt 3)}, pages={246-9} }