Immunotoxins containing saporin 6 and monoclonal antibodies recognizing plasma cell-associated antigens: effects on target cells and on normal myeloid precursors (CFU-GM).

@article{Barbieri1989ImmunotoxinsCS,
  title={Immunotoxins containing saporin 6 and monoclonal antibodies recognizing plasma cell-associated antigens: effects on target cells and on normal myeloid precursors (CFU-GM).},
  author={Luigi Barbieri and Angelo Dinota and Marco Gobbi and Pier Luigi Tazzari and Simonetta Rizzi and Andrea Bontadini and Roberto Massimo Lemoli and Sante Tura and Fiorenzo Stirpe},
  journal={European journal of haematology},
  year={1989},
  volume={42 3},
  pages={238-45}
}
Monoclonal antibodies 8A and 62B1, recognizing plasma cell-associated antigens, were covalently linked to saporin 6, a ribosome-inactivating protein similar to the A-chain of ricin. Both immunotoxins were tested on target human cell lines U266 and Raji, on non-target K562 cell line and on myeloid CFU-GM progenitors. The cloning efficiency and viability of target cells were strongly reduced by 8A-saporin 6 and 62B1-saporin 6 immunotoxins, with an ID50 up to 200,000-fold lower than free saporin 6… CONTINUE READING

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