Immunotherapeutic potential in guinea-pig tumor model of deoxyribonucleic acid from Mycobacterium bovis BCG complexed with poly-L-lysine and carboxymethylcellulose.

  title={Immunotherapeutic potential in guinea-pig tumor model of deoxyribonucleic acid from Mycobacterium bovis BCG complexed with poly-L-lysine and carboxymethylcellulose.},
  author={Tetsuro Kataoka and S Yamamoto and T. Yamamoto and Tohru Tokunaga},
  journal={Japanese journal of medical science \& biology},
  volume={43 5},
In vivo antitumor activity of a deoxyribonucleic acid fraction obtained from Mycobacterium bovis BCG (named MY-1) increased when it was complexed with poly-L-lysine (poly LL) solubilized by addition of carboxymethylcellulose (CMC). The complex of MY-1 and poly LL/CMC induced interferon in vivo at a low dose of MY-1 which alone exerted no IFN induction. With Line 10 hepatoma (L10) which is syngeneic with strain 2 guinea pigs, it was demonstrated that repeated intralesional injections of the… 
7 Citations

Modulation of interleukin-12 synthesis by DNA lacking the CpG motif and present in a mycobacterial cell wall complex

The results emphasize that M. phlei DNA associated with the cell wall complex makes a significant contribution to the overall immunomodulatory and anticancer activity of this mycobacterial cell wall preparation and that these activities are not correlated with the presence of CpG motifs.

Recombinant interleukin‐12 and interleukin‐18 antitumor therapy in a guinea‐pig hepatoma cell implant model

Interleukin (IL)‐12 and IL‐18 are secreted by myeloid cells activated with adjuvants such as Bacillus Calmette‐Guérin (BCG) cell wall and upregulate production of lymphocyte interferon‐γ, which leads to the induction of an antitumor gene program.

Mycobacteria-Based Vaccines as Immunotherapy for Non-urological Cancers

This review focuses on the preclinical and clinical assays using mycobacteria to treat non-urological cancers, providing a wide knowledge of the beneficial applications of these microorganisms to manipulate the tumor microenvironment aiming at tumor clearance.

Influence of Vehicles Used for Oral Dosing of Test Molecules on the Progression of Mycobacterium tuberculosis Infection in Mice

Corn oil significantly improved both the parameters and the progression of Mycobacterium tuberculosis infection in mice compared with water, whereas the other vehicles affected only physical parameters.

Influence of Vehicles Used for Oral Dosing of Test Molecules on the Progression of Mycobacterium tuberculosis Infection in Mice

Compared with water, corn oil significantly improved both sets of parameters, whereas the other vehicles affected only physical parameters.

PAMAM dendrimers: destined for success or doomed to fail? Plain and modified PAMAM dendrimers in the context of biomedical applications.

A brief critical review of the biomedical activities of these unmodified compounds and their most alluring derivatives, especially in the context of possible future perspectives of PAMAMs is prepared.

DNA preparations as potential therapeutic agents

DNA increased the mitotic activity of hematopoietic cells both in intact animals and in animals with experimental cytopenia, and was connected with elaboration of systems for directed transport of DNA incapsulated in liposomes containing cationic lipids.



Antitumor activity of the DNA fraction from Mycobacterium bovis BCG. II. Effects on various syngeneic mouse tumors.

MY-1 was equally effective in mice with or without presensitization with BCG, whereas BCG was much more effective in BCG-sensitized mice, suggesting that a delayed-type hypersensitivity reaction elicited by BCG protein is not required for the antitumor activity of MY-1.

In vivo augmentation of natural killer cell activity with a deoxyribonucleic acid fraction of BCG.

Antitumor activities of MY-1 were also abolished if the animals were pretreated with anti-asialo GM1 antiserum or carrageenan, suggesting that the activities can be ascribed mainly to activated NK cells.

Antitumor activity of deoxyribonucleic acid fraction from Mycobacterium bovis BCG. I. Isolation, physicochemical characterization, and antitumor activity.

A fraction extracted from Mycobacterium bovis strain BCG, which was composed of 70% DNA, 28.0% RNA, 1.3% protein, 0.20% glucose, and 0.1% lipid, was found to possess strong antitumor activity, suggesting that the DNA from BCG possessed strong antitUMor activity under certain conditions.

Immunotherapeutic potential in murine tumor models of polyinosinic-polycytidylic acid and poly-L-lysine solubilized by carboxymethylcellulose.

The systemic administration of multiple, nontoxic doses of polyinosinic-polycytidylic acid and poly-L-lysine solubilized by carboxymethylcellulose eradicated established experimental and spontaneous pulmonary metastases and suggest that the systemic Administration of poly(I,C)-LC may provide a successful adjuvant therapeutic modality against cancer metastasis.

Immunomodulatory effects in mice of polyinosinic-polycytidylic acid complexed with poly-L-lysine and carboxymethylcellulose.

The results indicate that poly(I,C)-LC has considerable potential as an immunotherapeutic agent, with the ability not only to induce macrophage and NK cell activation but also to stimulate specific cytotoxic T-lymphocytes.


Small quantities of the non-toxic residue of phenol-killed, acetone-washed, and methanol-extracted tubercle bacilli of the BCG strain conferred a high degree of resistance on mice against otherwise

[Phase I clinical study of MY-1, a new biological response modifier].

After MY-1 treatment, enhanced PPD skin reaction and increased OKT4/OKT8 ratio in peripheral lymphocyte subsets were observed which were statistically significant especially at a dose level of 3 mg, the optimal dose and schedule for phase II clinical study were considered.

Mycobacterial RNA. A comparison with intact mycobacteria for suppression of murine tumor growth.

The host's immunological system is a necessary component for inhibition of tumors by the authors' test materials since there is no tumor suppression in immunologically impaired mice.

Water-soluble and lipid-free fraction from BCG with adjuvant and antitumour activity.

  • I. Hiu
  • Biology
    Nature: New biology
  • 1972
A fully water-soluble lipid-free fraction from BCG is isolated, called MAAF, which is not toxic and stimulates cell-mediated and humoral immune responses and inhibited the development of tumours in mice.

In Situ Infiltration of Natural Killer‐Like Cells Induced by Intradermal Injection of the Nucleic Acid Fraction from BCG

Results indicate that the nucleic acid components of MY‐1 are responsible for the induced in situ infiltration of mononuclear cells that markedly resembled natural killer (NK) cells in their cytochemical characteristics and surface markers.