Immunostimulatory DNA Sequences Necessary for Effective Intradermal Gene Immunization

@article{Sato1996ImmunostimulatoryDS,
  title={Immunostimulatory DNA Sequences Necessary for Effective Intradermal Gene Immunization},
  author={Yukio Sato and Mark Roman and Helen Tighe and Delphine J Lee and Maripat Corr and Minh-Duc Nguyen and Gregg J. Silverman and Martin Lotz and Dennis A. Carson and Eyal Raz},
  journal={Science},
  year={1996},
  volume={273},
  pages={352 - 354}
}
  • Yukio Sato, M. Roman, +7 authors E. Raz
  • Published 19 July 1996
  • Biology, Medicine
  • Science
Vaccination with naked DNA elicits cellular and humoral immune responses that have a T helper cell type 1 bias. However, plasmid vectors expressing large amounts of gene product do not necessarily induce immune responses to the encoded antigens. Instead, the immunogenicity of plasmid DNA (pDNA) requires short immunostimulatory DNA sequences (ISS) that contain a CpG dinucleotide in a particular base context. Human monocytes transfected with pDNA or double-stranded oligonucleotides containing the… Expand
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Investigators have attempted to enhance the efficacy of DNA vaccines by co-injecting plasmids encoding immune stimulatory cytokines, such as IL-2 and GM-CSF, along with plasmid encoding the antigen. Expand
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Plasmid DNA vaccines are effective in the absence of IFNgamma.
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Preliminary studies suggest that the use of immunomodulatory cytokines with plasmid DNA vaccines may allow clinicians to tailor the resulting immune response to more closely resemble the correlates of protection for a given pathogen. Expand
Multivalent DNA-based immunization against hepatitis B virus with plasmids encoding surface and core antigens.
The immune response against hepatitis B surface and core antigens was evaluated by either coinoculation or independent intramuscular administration of pAEC compact DNA immunization vectors carryingExpand
Immunostimulatory effects of plasmid DNA and synthetic oligodeoxynucleotides
TLDR
It is established that plasmid DNA express their adjuvanticity as either double or single strands, and no differences were observed between modified and unmodified plasmids. Expand
Cytokine-FC fusion genes as molecular adjuvants for DNA vaccines.
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The approach to develop cytokine-Fc fusion genes as molecular adjuvants and will use GM-CSF as an example is outlined. Expand
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