Immunoselection of stably transformed MDCK cells expressing the vesicular stomatitis virus G-protein at the basolateral surface.

Abstract

We have used immunoisolation on a magnetic solid support for the positive selection of stable MDCK transformants which express VSV-G protein via genomic integration of a cloned cDNA. This method is a simple, inexpensive alternative to selection with a fluorescence-activated cell sorter. The G-protein is synthesized in the absence of other viral proteins and… (More)

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