Immunoproteomics of membrane proteins of Shigella flexneri 2a 2457T.

Abstract

AIM To screen the immunogenic membrane proteins of Shigella flexneri 2a 2457T. METHODS The routine two-dimensional polyacrylamide gel electrophoresis (2-DE) and Western blotting were combined to screen immunogenic proteins of S. flexneri 2a 2457T. Serum was gained from rabbits immunized with the same bacteria. Immunogenic spots were cut out from the polyacrylamide gel and digested by trypsin in-gel. Matrix-assisted laser desorption/ionization time of flight-mass spectrometry (MALDI-TOF-MS) was performed to determine the molecular weight of peptides. Electrospray ionization (ESI-MS/MS) was performed to determine the sequences of the interesting peptides. RESULTS A total of 20 spots were successfully identified from Coomassie brilliant blue stained gels representing 13 protein entries, 5 known antigens and 8 novel antigens. A hypothetical protein (YaeT) was detected, which might be a candidate target of vaccine. CONCLUSION Membrane proteins of S. flexneri 2a 2457T were successfully observed by 2-DE. Several known and novel antigens were identified by mass spectrum.

Cite this paper

@article{Ying2005ImmunoproteomicsOM, title={Immunoproteomics of membrane proteins of Shigella flexneri 2a 2457T.}, author={Tian-Yi Ying and Junjun Wang and Heng-Liang Wang and Er-Ling Feng and Kai-hua Wei and Liu-yu Huang and Pei-Tang Huang and Cui-fen Huang}, journal={World journal of gastroenterology}, year={2005}, volume={11 43}, pages={6880-3} }